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Fig. 3 | Virology Journal

Fig. 3

From: RUNX1 inhibits the antiviral immune response against influenza A virus through attenuating type I interferon signaling

Fig. 3

Overexpression of RUNX1 facilitates PR8 replication. A549 cells were transfected with vector plasmid pCMV-GFP or pCMV-RUNX1. a RUNX1 overexpression efficiency was detected by Western blot. b RUNX1 overexpression efficiency was detected by qRT-PCR. c These cells were infected with PR8 (MOI = 1) and collected at 3, 6, and 9 h.p.i. The viral NP and M1 protein were assessed by Western blot. d These cells were infected with PR8 (MOI = 1) and collected at 8 h.p.i. The viral NP and M RNA was assessed by qRT-PCR. e Culture supernatants were collected for viral titration by TCID50 assay at 24 h.p.i. Data are mean ± SD of three independent experiments. Significance is by unpaired T-test; *p < 0.05; **p < 0.01; ***p < 0.001

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