Fig. 1

Influenza virus A/Puerto Rico/8/34 (H1N1) (PR8) infection induced RUNX1 expression. a A549 cells were infected with PR8 at indicated MOIs and harvested at 12 h.p.i., protein levels of RUNX1, vial NP, and GAPDH were analyzed by Western blot. b A549 cells were infected with PR8 at indicated MOIs and harvested at 12 h.p.i., and RUNX1 and GAPDH mRNA levels were analyzed by qRT-PCR. c A549 cells were infected with IAV at an MOI of 1. Cells were harvested at 3, 6, and 12 h.p.i. and analyzed by Western blot with anti-RUNX1, anti-NP, and anti-GAPDH antibodies. d A549 cells were infected with PR8 at an MOI of 1. Cells were harvested at 3, 6, and 12 h.p.i. and RUNX1 and GAPDH mRNA levels were analyzed by qRT-PCR. e A549 cells were treated with 1 μg/ml poly(I:C) with (+) or without (−) transfection reagent lipofectamine 2000 for 12 h and collected for Western blot analysis of RUNX1. Data are mean ± SD of three independent experiments. Significance is by two-way ANOVA test; *p < 0.05, **p < 0.01