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Fig. 7 | Virology Journal

Fig. 7

From: The long non-coding RNA LNC_000397 negatively regulates PRRSV replication through induction of interferon-stimulated genes

Fig. 7

a PAMs were transfected with 50 nM NC siRNA or two distinct siRNAs against LNC_000397, 24 h after transfection total RNA was extracted from cell lysates. MX1, CXCL10, ISG15, RSAD2 were quantified by qRT-PCR at the indicated times. b PAMs and c PK15 cells were treated with IFN-α and TNF-α, 24 h after treatment, total RNAs were extracted and the LNC_000397 mRNA levels were determined by qRT-PCR. The bar represents the mean of three independent experiments. Expression levels were normalized to GAPDH

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