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Fig. 5 | Virology Journal

Fig. 5

From: The long non-coding RNA LNC_000397 negatively regulates PRRSV replication through induction of interferon-stimulated genes

Fig. 5

a and b Screening of lncRNAs that regulate PRRSV replication. PAMs were transfected with siRNAs targeting five up-regulated lncRNAs, 24 h after transfection cells were inoculated with a GSWW or b VR2332 with an MOI of 0.1. At 24 hpi, total RNAs were subjected to qRT-PCR analysis for PRRSV ORF7. c The knockdown efficiency of LNC_0003997 was determined by qRT-PCR. PAMs were transfected with 50 nM Negative Control (NC) siRNA or two distinct siRNAs against LNC_000397 for 24 h before infecting them with GSWW or VR2332 (MOI = 0.1). At 24 hpi, total RNAs were extracted for PRRSV ORF7 quantification using qRT-PCR. d and the supernatants were collected for TCID50 determination (e). The bar represents the mean of three independent experiments. Expression levels were normalized to GAPDH

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