Fig. 2
From: Design of a chimaeric antigen and its use in the detection of IgG antibodies against rubella virus
Western blot identification of induced expression of P6 with human serum. The constructed recombinant expression plasmid was transformed into BL21(DE3), and then induced by IPTG to obtain P6 in the form of inclusion body.Western blot was used to identify P6 with positive and negative anti-RV serum respectively, and the desired band was obtained in the positive serum. 1: Marker; 2. control (no IPTG induction); 3. expression product (IPTG induction: 2.5 h, 1 mM IPTG); 4. supernatant of expressed product; 5. marker; 6. control; 7. positive serum + P6; 8. negative serum + P6