Table 1 Primers used for the amplification of HPV16 and HPV52 L1, E6 and E7 genes
Primer name | Start codon | Sequence 5–3 | Amplicon size (bp) |
|---|---|---|---|
HPV16 L1 1F | 4636 | GACCAAGCTCCTTCATTAATTCCT | 1051 |
HPV16 L1 1R | 5686 | GGCATCAGAGGTAACCATAGAAC | |
HPV16 L1 2F | 5393 | GCTATGGACTTTACTACATTACAGGC | 971 |
HPV16 L1 2R | 6363 | TTTACAAGCACATACAAGCACATA | |
HPV16 E6 F | 7119 | TTATGCACCAAAAGAGAACTGCA | 502 |
HPV16 E6 R | 7620 | GGTGTATCTCCATGCATGATTACAGC | |
HPV16 E7 F | 7595 | GCTGTAATCATGCATGGAGATACACCT | 316 |
HPV16 E7 R | 4 | GCAGGATCAGCCATGGTAGATTAT | |
HPV52 L1 1F | 5457 | CCACTATGTCCATTGAGTCAGGTC | 1013 |
HPV52 L1 1R | 6469 | CCTGGCACAGGGTCACCTAAG | |
HPV52 L1 2F | 6205 | GTCCTCCCCTACAGCTCATTAA | 977 |
HPV52 L1 2R | 7181 | CACAGACAATTACCCAACAGAC | |
HPV52 E6 F | 31 | GGGTGTAACCGAAAACGG | 545 |
HPV52 E6 R | 575 | ATAGTTGCTTTGTCTCCACG | |
HPV52 E7 F | 354 | GGGAAAACATTAGAAGAG | 591 |
HPV52 E7 R | 944 | TTGTTTTTCTATTATTGCCTCTA |