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Table 2 Antigenic distance information of AH/17, FJ/20 and their mutants

From: The molecular determinants of antigenic drift in a novel avian influenza A (H9N2) variant virus

AH/17 and FJ/20 mutants

Distance from AH/17a

Distance from FJ/20a

Color in the antigenic mapb

AH/17

–

3.2

Red

FJ/20

3.2

–

Red

AH/17_Chim_II

3.3

3.0

Green

AH/17_Chim_III

4.1

2.8

Green

AH/17_Chim_IV

2.1

3.1

Green

AH/17_48Q/72E/73E/112K/246Hc

1.5

3.3

Green

AH/17_48Q/264T/265Kc

1.5

3.6

Green

AH/17_127D/135G

3.4

3.2

Violet

AH/17_179D/182R/183T

2.5

3.5

Violet

AH/17_127D/135G/145N/146R

3.9

2.7

Violet

AH/17_127D/135G/146R

3.6

3.1

Blue

AH/17_127D/135G/179D/182R/183T

2.7

2.5

Orange

AH/17_145N/146R/179D/182R/183T

3.1

3.3

Orange

FJ/20_Chim_I

2.9

0.4

Green

FJ/20_Chim_II

1.8

1.9

Green

FJ/20_Chim_III

0.6

2.6

Green

FJ/20_Chim_IV

3.1

1.2

Green

FJ/20_127S/135D

2.4

1.3

Violet

FJ/20_145T/146Q

2.3

0.9

Violet

FJ/20_179T/182T/183N

3.0

0.6

Violet

FJ/20_145T/146Q/179T/182T/183N

3.0

1.3

Orange

  1. aOne unit of antigenic distance is equal to a twofold difference in the HI assay
  2. bThe color indicates the colored dots in Figs. 4 and 5
  3. cThe positions on the antigenic map were shown in Additional file 2: Fig. S1