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Fig. 3 | Virology Journal

Fig. 3

From: A UV-LED module that is highly effective at inactivating human coronaviruses and HIV-1

Fig. 3

UV effectively inactivates viruses of varying titres. A Dilutions of HIV-1 IIIB was left untreated (UV-, grey bars), irradiated with UV (UV+, purple bars) for 30 s. Virus replication post-treatment was determined by measuring luminescence (RLU, relative light units) of a luciferase-based reporter cell line. Bars are mean ± SD of duplicate luminescence readings and are representative of three independent experiments. B Three MOIs of hCoV-229E were left untreated (UV-, grey bars) or irradiated with UV for 30 s (UV+, purple bars). Virus replication was determined by measuring the relative quantity (Rq) of hCoV-229E transcripts by qPCR in total RNA isolated from infected cells. Eukaryotic 18S rRNA was used for the endogenous control and mock-infected cells was used as the reference sample for Rq. Bars are mean ± SD of triplicate qPCR assays and are representative of two independent experiments

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