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Fig. 4 | Virology Journal

Fig. 4

From: Interleukin-17A pretreatment attenuates the anti-hepatitis B virus efficacy of interferon-alpha by reducing activation of the interferon-stimulated gene factor 3 transcriptional complex in hepatitis B virus-expressing HepG2 cells

Fig. 4

IL-17A pretreatment inhibited IFN-α-induced STAT1/2 phosphorylation and IRF9 expression. A HepG2-HBV1.3 cells were pretreated with or without 50 ng/ml IL-17A for 24 h, followed by co-treatment with or without 1000 IU/ml IFN-α for 24 or 48 h. Cell lysates were collected for assay of p-STAT1, T-STAT1, p-STAT2, T-STAT2 and IRF9 protein by western blotting. Relative expression was shown as fold-change compared to group of IFN-α treatment alone. Data were shown as mean ± standard deviation (SD) (error bars) from at least 3 independent experiments. p < 0.05 is considered statistically significant. *p < 0.05, **p < 0.01 between two indicated groups. B HepG2-HBV1.3 cells were pretreated with or without 50 ng/ml IL-17A for 24 h, followed by co-treatment with or without 1000 IU/ml IFN-α for 48 h. Cell slides were processed for immunofluorescent staning and fluorescence microscopy imaging. The merge images displayed positive expression of p-STAT1, p-STAT2 and IRF9 (green) in cell nucleus (in blue by DAPI staining)

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Virology Journal

ISSN: 1743-422X