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Fig. 1 | Virology Journal

Fig. 1

From: Construction of a quadruple gene-deleted vaccine confers complete protective immunity against emerging PRV variant challenge in piglets

Fig. 1

Schematic diagrams of the PRV GDFS-delgI/gE/US9/US2 strain. A Diagram of the PRV gI/gE/US9/US2 gene deletion. The transfer plasmid pSK-gIL-US2R was constructed for homologous recombination with co-transfection of two sgRNAs and PRV GDFS genome. B Identification of the plaque-purified viruses by PCR with specific primers (gIF and US2R). PRV GDFS was used as the positive control (P), and DMEM was used as the negative control (N)

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Virology Journal

ISSN: 1743-422X