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Fig. 3 | Virology Journal

Fig. 3

From: Antigenic and immunogenic properties of recombinant proteins consisting of two immunodominant African swine fever virus proteins fused with bacterial lipoprotein OprI

Fig. 3

Expression of costimulatory and antigen-presenting molecules by DCs induced by recombinant proteins. BMDCs (106/well) were incubated with p30 + modified p54, PM, OPM or OPMT (10 μg/mL each) for 24 h at 37 °C under 5% CO2. RPMI-1640 complete medium and LPS (0.1 µg/mL) served as negative and positive controls, respectively. After staining with anti-CD11c, anti-CD40, anti-CD80, anti-CD86 or anti-MHC-II fluorescent antibodies, the cells were gated on the CD11c-positive population to analyze expression of CD40, CD80, CD86 and MHC-II by flow cytometry. The percentages of CD40 (A), CD80 (B), CD86 (C) and MHC-II (D) positive cells in each group were calculated. The graph bars represent mean ± SD (n = 3); ns = P > 0.05; *** P < 0.001

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Virology Journal

ISSN: 1743-422X