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Fig. 2 | Virology Journal

Fig. 2

From: Antigenic and immunogenic properties of recombinant proteins consisting of two immunodominant African swine fever virus proteins fused with bacterial lipoprotein OprI

Fig. 2

Immunoblot analysis of the recombinant proteins. A The recombinant proteins were subjected to western-blotting with 1:5000 diluted anti-polyhistidine monoclonal antibody as primary antibody and 1:5000 diluted goat anti-mouse peroxidase conjugated secondary antibody. B Western blotting confirmation of recombinant proteins using 1:300 diluted anti-ASFV swine serum as primary antibody and 1:5000 diluted goat anti-pig peroxidase conjugated secondary antibody. Lane M: molecular weight markers; lane 1, p30; lane 2, modified p54; lane 3, PM; lane 4, OPM; lane 5, OPMT; lane 6, bovine serum albumin

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