The phosphatase and tensin homolog gene inserted between NP and P gene of recombinant Newcastle disease virus oncolytic effect test to glioblastoma cell and xenograft mouse model

Table 1 Primers for cDNA construction of NDV containing PTEN CDS

Gene	Direction	Sequence (5′ → 3′)	Size (bp)
Kozak PTEN for Position “1” (1st PCR)	Forward	GCCACCATGACAGCCATCATCAAAG	1221
Kozak PTEN for Position “1” (1st PCR)	Reverse	AGAAGCGGCCGCGGCCGGCCACCATGACAGCC	1221
Fse I Kozak PTEN for Position “1” (2nd PCR)	Forward	GCCTCAGACTTTTGTAATTTGTGTATG	1258
Fse I Kozak PTEN for Position “1” (2nd PCR)	Reverse	GTTGGACCTTGGTATGGCCGGCCTCAGACTTTTGTAATTTGTG	1258
Kozak PTEN for Position “2” (1st PCR)	Forward	GCCACCATGACAGCCATCATCAAAG	1221
Kozak PTEN for Position “2” (1st PCR)	Reverse	CGCTCAGACTTTTGTAATTTGTGTA	1221
Not I Kozak PTEN for Position “2” (2nd PCR)	Forward	ATACGGGTAGAAGCGGCCGCCACCATGACAGCC	1254
Not I Kozak PTEN for Position “2” (2nd PCR)	Reverse	TGGACCTTGGGCGGCCGCTCAGACTTTTGTAATTTG	1254

ISSN: 1743-422X