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Fig. 1 | Virology Journal

Fig. 1

From: The PB1 gene from H9N2 avian influenza virus showed high compatibility and increased mutation rate after reassorting with a human H1N1 influenza virus

Fig. 1

Viral RNPs functional assay of recombinant polymerase complex. The polymerase activities of the human WSN-H1N1 RNP, the recombinant RNPs containing PB2, PB1, PA or NP genes derived from the avian A2093-H9N2 strain, and the avian A2093-H9N2 RNP were measured using the Dual-Luciferase Assay System. Firefly luciferase activity was measured to determine polymerase activity, and Renilla luciferase activity was used as an internal control for monitoring transfection efficiency. The Y axis displayed the Fluc (firefly) /Rluc (Renilla) ratios. Experiments were repeated three times, mean and SD were applied. Multiple t-test with a = 0.05. *significant difference

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Virology Journal

ISSN: 1743-422X