Table 2 Primers and probes used for the detection and characterisation of PERV

Primers and probes for ddPCR

PERV pol

PERV pol—forward

CGA CTG CCC CAA GGG TTC AA

HM159246

3568–3587

Yang et al. [31]

PERV pol—reverse

TCT CTC CTG CAA ATC TGG GCC

3803–3783

PERV pol—probe

6FAM-CAC GTA CTG GAG GAG GGT CAC CTG-BHQ1

3678–3655

pGAPDH

pGAPDH—forward

TTC ACT CCG ACC TTC ACC AT

396823

3951–3970

pGAPDH—reverse

CCG CGA TCT AAT GTT CTC TTT C

4022–4001

pGAPDH—probe

HEX-CAG CCG CGT CCC TGA GAC AC-BHQ1

3991–3972

pActin

pActin—forward

TAA CCG ATC CTT TCA AGC ATT T

pActin—reverse

TGG TTT CAA AGC TTG CAT CAT A

pActin—probe

HEX-CGT GGG GAT GCT TCC TGA GAA AG-BHQ1

Primers to detect PERV-C

PERV-C

PERV EnvC—forward

CCC CAA CCC AAG GAC CAG

AM229312

9601–9618

Kaulitz et al. [46]

PERV EnvC—reverse

AAG TTT TGC CCC CAT TTT AGT

9692–9672

PERV EnvC—probe

6FAM-CTC TAA CAT AAC TTC TGG ATC AGA CCC-BHQ1

9626–9652

Primers to detect PERV-A/C

PERV-A/C (380 bp amplicon)

PERV-A-VRB—forward

CCT ACC AGT TAT AAT CAA TTT AAT TAT GGC

AY570980.1

6129–6158

Dieckhoff et al. [14]

PERV-C reverse

TAT GTT AGA GGA TGG TCC TGG TC

6451–6473

PERV-A/C (1200 bp amplicon)

PERV-A-VRB—forward

CCT ACC AGT TAT AAT CAA TTT AAT TAT GGC

AY570980.1

6129–6158

Wood et al. [10]

PERV-C-TMR

CTC AAA CCA CCC TTG AGT AGT TTC C

7370–7395

Primers for LTR amplification

PERV LTR

PK34

AAA GGA TGA AAA TGC AAC CTA ACC

Y17012

3–26

Czauderna et al. [45]

PK26

ACG CAC AAG ACA AAG ACA CAC GAA

1134-1111