Fig. 7
Detection of viral proteins by Western blot analysis. A Cell lysates from 293 cells infected with PERV-F and with PERVi were analysed in 1, 1:10 and 1:100 dilution, using goat serum 62 to detect the SU-Env gp70, goat serum 30 to detect the p27Gag and goat serum 16 to detect the transmembrane envelope protein p15E. PERVi is a new infection (i) of PERV-50 [24]. The sera were used 1:1000, an alkaline phosphatase conjugates donkey anti-goat serum was used 1:2000. An unstained protein ladder was used and the molecular weights in kDa were indicated with lilac arrows. B Virus pellets from supernatants of 293 cells producing PERV-F at day 52 and 65 and 293 cells producing PERVi were analysed using goat serum 62 and 30, recombinant gp70 (molecular weight 54) and recombinant p27 were used as positive control. A prestained protein ladder was used and the molecular weights in kDa were indicated with lilac arrows