Fig. 4

Isolation and identification of EHV-8. The RK-13 cells were inoculated with supernatant of EHV-8-positive placenta (right panel) or mock control (left panel). a A total of 48 h post-infection, the CPE was observed using microscopy. Scale bars, 100 µm. b Identification of EHV-8 isolate by IFA. CPE-positive RK-13 cells and mock control cells were fixed with 75% alcohol. Images represent the subcellular locations of EHV-8 proteins using indirect immunofluorescence detection using anti-EHV-8 mouse serum, and the corresponding DyLight 594-conjugated secondary antibodies. Cells were imaged by Leica DMi8. Scale bars, 50 µm. c PCR detection of the EHV-8 ORF70 genes from a different group. The DNA was extracted from these cells. PCR products were electrophoresed in a 1% agarose gel. Marker (lane M) was included on the left, 1 represents negative control, 2 represents mock control RK-13 cell, 3 represents the CPE positive RK-13 cells