Fig. 4
From: 3C protease of enterovirus 71 cleaves promyelocytic leukemia protein and impairs PML-NBs production
EV71 3C protease cleaves PML to maintain virus replication. The control (HeLa-ScrambledKO), and the stable PML-knockout cells (HeLa-PMLKO) were cultured in 12-well plates to 60% confluency and transfected with Flag-PMLIV (lane 2, 5), Flag-PMLIV (Q430A/Q444A) (lane 3, 6), or vectors (lane 1, 4). After 24 h, all cells were infected with EV71 at the multiplicity of infection (MOI) of 1 for 8 h. EV71 capsid protein VP1, and PMLIV or its variant were analyzed by Western blotting. Tubulin was used as the protein loading control