Fig. 3
From: 3C protease of enterovirus 71 cleaves promyelocytic leukemia protein and impairs PML-NBs production
Q430–A431 and Q444–S445 are the sites of PMLIV cleavage by 3C. A The amino acids sequence of PMLIV protein (NCBI Reference Sequence: NP_002666.1). The predicted cleavage sites of EV71 3C protease were marked in red. The Hash sign represents the terminator. B Co-transfection analysis of PMLIV variants and 3C (lane 2, 5, 8) or 3C (E71A) (lane 3, 6, 9) or vectors (lane 1, 4, 7) in HEK293T cells. After 36 h, lysates were subjected to Western blotting. C Co-transfection analysis of PMLIV (Q430A/Q444A) dual-mutation variant and 3C (lane 2, 5) or 3C (E71A) (lane 3, 6) or vectors (lane 1, 4) in HEK293T cells. After 36 h, lysates were tested by Western blotting. D Schematic demonstration of the identified cleavage sites of 3C in PMLIV. E HEK293T cells were transfected with Myc-3C and/or Flag-PMLIV (Q430A/Q444A) as indicated for 36 h. The cell lysates were subjected to Flag IP, and samples of both cell lysates and immunoprecipitates were tested with the indicated antibodies