Table 1 Nested PCR primers designed based on ORF1 for screening positive samples
From: Viral metagenomics reveals two novel anelloviruses in feces of experimental rats
Virus | Primer | Sequence | Amplified product (bp) | Tm (℃) |
|---|---|---|---|---|
ratane153-zj1 | WF | CTTCTGGTGGAGCACAGAGG | 536 | 48 |
| Â | WR | AATACCAAGCAGCAGGCCAT | Â | Â |
| Â | NF | ATCATTCACAGAAGCGGCCA | 312 | 53 |
| Â | NR | AGCAAGGTCTCGTATTCCGC | Â | Â |
ratane153-zj2 | WF | GTTCGTGACCCAGACAACCT | 492 | 48 |
| Â | WR | AGATTCCTGCCTCCCCATCT | Â | Â |
| Â | NF | AGCAGAGATAGGGTAGGGCC | 277 | 55 |
| Â | NR | GCTCGGCTTCTGACAGAGTA | Â | Â |