Skip to main content

Table 1 Primers used in this study

From: The interactions of ZDHHC5/GOLGA7 with SARS-CoV-2 spike (S) protein and their effects on S protein’s subcellular localization, palmitoylation and pseudovirus entry

Name

Sequence(5'to 3')a

Usage

S-F2

CTAGCGTTTAAACTTAAGCTTATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGCGTGAACCTGACCACA (HindIII)

pcDNA3.1-S-HA, pcDNA3.1-SC10A-HA/Co-IP, ABE

S-HA-R

TGCTGGATATCTGCAGAATTCTTAAGCGTAATCTGGAACATCGTATGGGTACATGGTGTAGTGCAGCTTCACGCC (EcoRI)

SA1235C-F

TATCGTGATGGTGACCATCATGCTGTGCGCTATGACATCC

pcDNA3.1-SA1235C-HA/ABE

SA1235C-R

GGATGTCATAGCGCACAGCATGATGGTCACCATCACGATA

SA1236C-F

TATCGTGATGGTGACCATCATGCTGGCCTGTATGACATCC

pcDNA3.1-SA1236C-HA/ABE

SA1236C-R

GGATGTCATACAGGCCAGCATGATGGTCACCATCACGATA

SA1240C-F

CATCATGCTGGCCGCTATGACATCCTGCGCTTCTGCCCTG

pcDNA3.1-SA1240C-HA/ABE

SA1240C-R

CAGGGCAGAAGCGCAGGATGTCATAGCGGCCAGCATGATG

SA1241C-F

CATCATGCTGGCCGCTATGACATCCGCCTGTTCTGCCCTG

pcDNA3.1-SA1241C-HA/ABE

SA1241C-R

CAGGGCAGAACAGGCGGATGTCATAGCGGCCAGCATGATG

SA1243C-F

GCTGGCCGCTATGACATCCGCCGCTTCTTGCCTGAAGGGC

pcDNA3.1-SA1243C-HA/ABE

SA1243C-R

GCCCTTCAGGCAAGAAGCGGCGGATGTCATAGCGGCCAGC

SA1247C-F

GACATCCGCCGCTTCTGCCCTGAAGGGCTGCGCTAGCGCT

pcDNA3.1-SA1247C-HA/ABE

SA1247C-R

AGCGCTAGCGCAGCCCTTCAGGGCAGAAGCGGCGGATGTC

SA1248C-F

GACATCCGCCGCTTCTGCCCTGAAGGGCGCCTGTAGCGCT

pcDNA3.1-SA1248C-HA/ABE

SA1248C-R

AGCGCTACAGGCGCCCTTCAGGGCAGAAGCGGCGGATGTC

SA1250C-F

TCTGCCCTGAAGGGCGCCGCTAGCTGTGGCTCCGCCGCTA

pcDNA3.1-SA1250C-HA/ABE

SA1250C-R

TAGCGGCGGAGCCACAGCTAGCGGCGCCCTTCAGGGCAGA

SA1253C-F

TCTGCCCTGAAGGGCGCCGCTAGCGCTGGCTCCTGCGCTA

pcDNA3.1-SA1253C-HA/ABE

SA1253C-R

TAGCGCAGGAGCCAGCGCTAGCGGCGCCCTTCAGGGCAGA

SA1254C-F

CTGAAGGGCGCCGCTAGCGCTGGCTCCGCCTGTAAGTTTG

pcDNA3.1-SA1254C-HA/ABE

SA1254C-R

CAAACTTACAGGCGGAGCCAGCGCTAGCGGCGCCCTTCAG

ZDHHC5-F

CTTGGTACCGAGCTCGGATCCATGCCCGCAGAGTCTGGAAAG (BamHI)

pcDNA3.1-ZDHHC5-3Flag/co-IP

ZDHHC5-3Flag-R

TGCTGGATATCTGCAGAATTCTCACTTATCGTCGTCATCCTTGTAATCGATCTTATCGTCGTCATCCTTGTAATCTCCCTTATCGTCGTCATCCTTGTAATCCACCGAAATCTCATAGGTGG (EcoRI)

GOLGA7-F

CTTGGTACCGAGCTCGGATCCATGAGGCCGCAGCAGGCGCC (BamHI)

pcDNA3.1-GOLGA7-3Flag/co-IP

GOLGA7-3Flag-R

TGCTGGATATCTGCAGAATTCTTACTTATCGTCGTCATCCTTGTAATCGATCTTATCGTCGTCATCCTTGTAATCTCCCTTATCGTCGTCATCCTTGTAATCTCTTCCACTGCTCATGCCTC (EcoRI)

ZDHHC5-C134S-F

TGTGGAGGAATTTGATCATCACTCCCCCTGGGTGAATAAC

pcDNA3.1-ZDHHC5-C134S-3Flag/co-IP

ZDHHC5-C134S-R

GTTATTCACCCAGGGGGAGTGATGATCAAATTCCTCCACA

ZDHHC5PDZ-3Flag-R

TGCTGGATATCTGCAGAATTCTCACTTATCGTCGTCATCCTTGTAATCGATCTTATCGTCGTCATCCTTGTAATCTCCCTTATCGTCGTCATCCTTGTAATCGGTGGTACCACCAACCCCTG (EcoRI)

pcDNA3.1-ZDHHC5PDZ-3Flag/co-IP

ZDHHC5PDZ-R

TTATCTAGATCCGGTGGATCCTCAGGTGGTACCACCAACCCCTG (BamHI)

pDsRed2-ZDHHC5PDZ/localization

S-F

TCAGATCTCGAGCTCAAGCTTATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGCGTGAACCTGACCACA (HindIII)

pEGFP-S/localization

S-R

CACCATGGTGGCGATGGATCCGGTGTAGTGCAGCTTCACGCC (BamHI)

SC10A-R

CACCATGGTGGCGATGGATCCGGTGTAGTGCAGCTTCACGCCCTTCAGCACTGGCTCGGAATCGTCCTCATCAAACTTAGCGGCGGAGCCAGCGCTAGCGGCGCCCTTCAGGGCAGAAGCGGCGGATGTCATAGCGGCCAG (BamHI)

pEGFP-SC10A/localization

ZDHHC5-F2

TCGAGCTCAAGCTTCGAATTCTATGCCCGCAGAGTCTGGAAAG (EcoRI)

pDsRed2-ZDHHC5/localization

ZDHHC5-R2

TTATCTAGATCCGGTGGATCCTCACACCGAAATCTCATAGG (BamHI)

GOLGA7-F2

TCGAGCTCAAGCTTCGAATTCTATGAGGCCGCAGCAGGCGCC (EcoRI)

pDsRed2-GOLGA7/colocalization

GOLGA7-R2

TTATCTAGATCCGGTGGATCCTTATCTTCCACTGCTCATGC (BamHI)

ZDHHC5-sgRNA-F

CACCGGAACCACGTGAAATCCCGTG

lentiCRISPRv2-ZDHHC5 sgRNA/gene editing

ZDHHC5-sgRNA-R

AAACCACGGGATTTCACGTGGTTCC

ZDHHC5-F3

TGTATTGGTCGCCGGAACTA

PCR

ZDHHC5-R3

CAAGATCACGCCACTGGATG

ZDHHC5-seq-F

CACGTATCCTTCAAGGCC

Sequencing

GOLGA7-sgRNA-F

CACCGGGCGGCCAGTCATATCTCGA

lentiCRISPRv2-GOLGA7 sgRNA/gene editing

GOLGA7-sgRNA-R

AAACTCGAGATATGACTGGCCGCCC

GOLGA7-F3

CATTGCCTGTTCTGCTTGCA

PCR

GOLGA7-R3

CCCTCATGCCCAAAGATGGT

GOLGA7-seq-F

GTTTTGGTAACTTAGGCCCAG

Sequencing

APT1-F

CTTGGTACCGAGCTCGGATCCATGTGCGGCAATAACATGTC (BamHI)

pcDNA3.1-APT1-3Flag/co-IP

APT1-3Flag-R

TGCTGGATATCTGCAGAATTCTCACTTATCGTCGTCATCCTTGTAATCGATCTTATCGTCGTCATCCTTGTAATCTCCCTTATCGTCGTCATCCTTGTAATCATCAATTGGAGGTAGGAGTT (EcoRI)

APT2-F

CTTGGTACCGAGCTCGGATCCATGTGTGGTAACACCATGTC (BamHI)

pcDNA3.1-APT2-3Flag/co-IP

APT2-3Flag-R

TGCTGGATATCTGCAGAATTCTCACTTATCGTCGTCATCCTTGTAATCGATCTTATCGTCGTCATCCTTGTAATCTCCCTTATCGTCGTCATCCTTGTAATCGACAGGAGGCAGCAGCTTCT (EcoRI)

  1. aSequences of restriction sites are underline