Fig. 6

The effects of ZDHHC5 or GOLGA7 knockout on S protein palmitoylation and pseudovirus entry. a HEK293T, HEK293T-GOLGA7^KO and HEK293T-ZDHHC5^KO cells were transfected with plasmid expressing S-HA for 48 h. The palmitoylation levels of S protein were measured by ABE assays. b Cells were infected with an equal number of SARS-CoV-2 pseudotyped virions and the entry efficiencies were quantified at 48 hpi by measuring luciferase activity (in relative light units, RLU) (n = 3). ZDHHC5 or GOLGA7 knockout in A549 and Hela cells significantly reduced pseudovirus infection. Error bars indicate standard deviations. ** represents P < 0.01