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Fig. 2 | Virology Journal

Fig. 2

From: The interactions of ZDHHC5/GOLGA7 with SARS-CoV-2 spike (S) protein and their effects on S protein’s subcellular localization, palmitoylation and pseudovirus entry

Fig. 2

Analyzing interactions between SARS-CoV-2 S, SC10A and ZDHHC5, its mutants or GOLGA7 by co-IP. a Interaction between SARS-CoV-2 S protein and ZDHHC5 or GOLGA7. Cell lysates and immunoprecipitated protein complexes (IP) from HEK293T cells cotransfected with indicated plasmids S/pcDNA3.1, S/ZDHHC5 and S/GOLGA7 were subjected to Western blot analysis using anti-HA and anti-Flag antibodies. Cells lysates and IP showed the bands of S-HA, S2-HA, ZDHHC5-3Flag (81 kDa) and GOLGA7-3Flag (19 kDa). b Interaction between SC10A and ZDHHC5 or GOLGA7. Cell lysates and IP from HEK293T cells cotransfected with the indicated plasmids (SC10A/pcDNA3.1, SC10A/ZDHHC5 and SC10A/GOLGA7) were subjected to Western blot analysis. c Interaction between S protein and ZDHHC5-C134S (C134S) or ZDHHC5PDZ (PDZ) by co-IP. Cell lysates and IP from HEK293T cells cotransfected with the indicated plasmids (S/pcDNA3.1, S/C134S and S/PDZ) were subjected to Western blot analysis

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