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Table 1 Primers used in the qRT-PCR for the the viral RNA detection of transcriptome samples and validation of the RNA-seq

From: Transcriptional responses of Daphnis nerii larval midgut to oral infection by Daphnis nerii cypovirus-23

No

Primer name

Primer sequence (5' to 3')

Tm (°C)

Gene id

Target gene

 

S1-RTPCR-F

GTGCTGATGGTCTGCTAA

49.6

N/A

DnCPV S1

 

S1-RTPCR-R

TGATTGATGACGACATTGAG

51.5

  
 

S10-RTPCR-F

GTCCGCCAATACTCTCAG

52.6

N/A

DnCPV S10

 

S10-RTPCR-R

CGTAGTCCATCGTCAATCA

51.3

  

1

CASP8-F

ACTGGAGAAGACTATGAGGTTA

51.5

TRINITY_DN10280_c0_g1_i1_3

CASP8

2

CASP8-R

ACGCTGTCATCTTGGCTAA

53.7

  

3

CYP6AB13-F

GATTCACACCAGCATTCAG

51.0

TRINITY_DN11437_c0_g1_i1_6

CYP6AB13

4

CYP6AB13-R

CAGTCGTATATCTCGCCATA

50.5

  

5

CYP6B45-F

GCGATACCGAACCAGAAC

53.4

TRINITY_DN12532_c0_g7_i1_1

CYP6B45

6

CYP6B45-R

ATTGGCAGTAAGTGTGAGTT

51.0

  

7

DHRS4-F

TCTTCTATCGCCGCATATCA

52.8

TRINITY_DN12896_c1_g2_i3_3

DHRS4

8

DHRS4-R

CACCACCTCATTAGCAATCG

53.5

  

9

PNLIP-F

CACCTCGTAGACTTGGAAGA

53.5

TRINITY_DN12381_c0_g2_i1_6

PNLIP

10

PNLIP-R

GTTAGCGTTGCCATTGACA

53.2

  

11

PRSS1_2_3-F

CCTGGAAGATGGCGTGTT

55.4

TRINITY_DN10836_c0_g5_i1_6

PRSS1_2_3

12

PRSS1_2_3-R

TCGGCGGTAATTCGGTTAT

53.5

  

13

RDH12-F

GTCTAATCGTCCGCTATTGAG

52.5

TRINITY_DN14445_c0_g1_i1_3

RDH12

14

RDH12-R

CTGTAGGTGAAGATTGCCATT

52.2

  

15

SCARB1-F

AACACAACAAGAGGCATCAC

53.0

TRINITY_DN14140_c0_g1_i1_6

SCARB1

16

SCARB1-R

GTCGTCGGTTCAATATCCATAA

51.7

  

17

SLC46A1-F

TGGAACGACACGACAAGT

53.7

TRINITY_DN8071_c0_g1_i2_5

SLC46A1

18

SLC46A1-R

CAACAGAGTGCGAACAGTATA

51.7

  

19

SLC52A3-F

AAGCGATTGTGGAAGATGTC

52.5

TRINITY_DN11521_c0_g1_i2_4

SLC52A3

20

SLC52A3-R

CGGCATACACGAGTACGA

54.4

  

21

ABCA3-F

CGATATACGCCGCAAGTAAG

53.3

TRINITY_DN12365_c0_g1_i6_2

ABCA3

22

ABCA3-R

GCAGTTCTCTACATTCAGTTGA

51.8

  

23

ABCC4-F

AGTGGATGGAAGGTTGGAAT

53.3

TRINITY_DN11997_c1_g1_i24_2

ABCC4

24

ABCC4-R

CGGCTCTTGTGGTATAATTGA

51.9

  

25

CYP6B6-F

GGACTATTGTTGGCGAATC

50.7

TRINITY_DN13898_c0_g1_i1_4

CYP6B6

26

CYP6B6-R

TTGTGGAAGAAGACGATGT

50.5

  
 

GAPDH-F

TATGTTCGTTGTCGGAGTTA

50.1

TRINITY_DN5984_c0_g1_i2_2

GAPDH

 

GAPDH-R

TAGCAGTAGTGGCGTGTA

52.4

  

27

LYPLA3-F

ACATCCACGACACAAGACTA

52.8

TRINITY_DN10250_c0_g1_i1_1

LYPLA3

28

LYPLA3-R

GACCGATAATGAACTCCTGAAT

51.5

  

29

NTE-F

CAGCCTGGAAGGTAAGTAGT

53.6

TRINITY_DN14343_c0_g2_i1_4

NTE

30

NTE-R

CTCATAGACGAGCGACAGT

53.8

  

31

UGT-F

GCATTCATTCAAGTCCATCAG

51.3

TRINITY_DN14215_c0_g5_i7_5

UGT

32

UGT-R

GCCTCCATCAATAATCACCAA

52.2

  

33

DnRPL13-F

GAACTATTGGCATTGCTGTTG

52

TRINITY_DN4717_c0_g1_i2_3

RPL13

34

DnRPL13-R

TCCTCCTCATTGGCTTCAC

54.5

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