Fig. 5

Validation of RNA-seq profiles by real-time qPCR. To validate the RNA-seq data, the relative mRNA levels of 17 selected DEGs in the DnCPV_1 sample were examined by qPCR; The mRNA levels by qPCR are presented as the fold change compared with the Mock_1 sample after normalization against RPL13. The relative expression levels from the RNA-seq analysis were calculated as RPKM values. Error bars show mean ± SEM