Fig. 1
From: Advances in designing Adeno-associated viral vectors for development of anti-HBV gene therapeutics
AAV genome modifications. A A native single stranded AAV (ssAAV) genome, about 4.7 kb in size, customised for hepatic-specific transgene expression with use of liver-specific promoters such as mouse Transthyretin (TTR) or human TTR promoters. The cis-regulatory modules (CRM) to enhance expression and polyadenylation signal (pA) are also indicated. B Oversized AAV vector genome (bigger than 4.7.kb). C Dual overlapping AAV genomes with 5′ and 3′ transgene splits are indicated by yellow or grey boxes respectively. Pink boxes and a cross indicate homologous regions (HR) and a homologous recombination site respectively. D Dual trans-splicing AAV genomes. Splicing donor (SD) and splicing acceptor (SA) sequence indicated downstream or upstream of the transgene splits. The cross indicate the site for concatemerisation. E Dual hybrid AAV genomes. Highly homologous recombineering (HHR) region is indicated by pink boxes. F Two component vectors, each expressing Cas 9 or sgRNA. G A self-complementary AAV (scAAV) genome with half the size (about 2.35 kbp)