Fig. 5
From: Hepatitis B virus polymerase-specific T cell epitopes shift in a mouse model of chronic infection
Phenotypes and Functions of Hepatic PolN-Specific CD8+ T Cells. a C57Bl/6 mice (n = 5) were injected with nothing, 1010 or 1011vg of AAV8-1.3HBV. They were vaccinated 4 weeks later with 5 × 109 vp of AdC6PolN vector. Lymphocytes were isolated from individual livers 8 weeks later. Liver lymphocytes from naive mice served as controls. The left graph shows frequencies of CD8+ T cells over all live single lymphoid cells. The middle graph shows frequencies of tetramer+CD8+ T cells over all CD8+ T cells. The left graph shows frequencies of CD8+ T cells producing IFN-γ in response to the PolN peptide pool. Lines with stars above indicate significant differences by multiple t-test. b Cells were stained with antibodies to the indicated markers and gated on either CD44-tetramer-CD8+ T cells (naïve) or tetramer+CD8+ T cells. Graphs show the mean fluorescent intensity of the dyes linked to antibodies to the indicated markers. Lines with stars above indicate significant differences by Tukey's multiple comparisons test