Fig. 4

The effect of AAV8-1.3HBV on the breadth of AdC6-gDPolN-induced CD8⁺ T cell responses. a C57Bl/6 mice (n = 5) were injected with no, 10¹⁰ or 10¹¹vg of AAV8-1.3HBV vector. They were vaccinated 4 weeks later with 5 × 10¹⁰vp of the AdC6-gDPolN vaccine. Eight weeks later CD44⁺CD8⁺ T cells from spleens were probed for IFN-γ production in response to the individual peptides of the PolN sequence. The line at 0.1 reflects the cut-off for positive samples. b shows the relative distribution of responses to individual peptides to the total response. The pop-out slices highlight peptides that were not recognized by CD8⁺ T cells from mice that only received the vaccine. Legends indicate the peptides that scored positive