Fig. 3

CHB model. a C57Bl/6 mice (n = 3) were injected with 10¹⁰ or 10¹¹vg of the AAV8-1.3HBV vector. Sera collected at 2 weeks after injection were screened for HBV genome copies. b The experiment was repeated with 10¹⁰vg of AAV8-1.3HBV and sera from 9 mice were tested for 20 weeks. Data for different PCR runs were normalized to the first set of data obtained with week 2 sera, which were tested in each PCR run. c Mice (n = 5) were injected with 3 × 10¹¹ vg of AAV8-1.3HBV. Sera were harvested at baseline and at different times after injection and tested by a direct ELISA for HBsAg (left graph) or a competition ELISA for HBcAg (right graph). Sera from naïve mice serves as negative controls. Samples provided by the kits served as positive controls. Lines with stars above indicate significant differences by one-way ANOVA. d C57Bl/6 mice (n = 5) were injected with 10¹⁰vg of AAV8-1.3HBV vector. Together with naïve mice they were vaccinated 4 weeks later with 5 × 10⁹vp of the AdC6-gDPolN vector. At the indicated times after vaccination mice were bled and PBMCs were tested for PolN-specific CD8⁺ T cells. PBMCs from naïve mice served as controls. Lines with stars above indicate significant differences by two-way ANOVA