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Fig. 2 | Virology Journal

Fig. 2

From: Hepatitis B virus polymerase-specific T cell epitopes shift in a mouse model of chronic infection

Fig. 2

CD8+ T cell responses in different mouse strains. af Mice (n = 5) were injected with of 5 × 1010 vp (C57Bl/6 and BALB/c mice) or 5 × 10vp (HLA-A2 tg mice) of AdC6-gDPolN [a] AdC6-gDPolC [b] or AdC6-gDCore [c]. PBMCs were tested 2 (AdC6-gDPolN, AdC6-gDPolC) or 6 (AdC6-gDCore) weeks later by ICS for frequencies of HBV-specific CD8+ T cells. Naïve mice served as control. Lines with stars above indicate significant differences by t-tests. df Splenocytes of mice were tested ~ 2 months after immunization upon stimulation with peptide pools. Splenocytes were pooled from 5 mice and data are shown as frequencies over CD44+CD8+ cells. The dotted line at 0.1% reflects the experimental cut-off for positive samples. CD44+CD8+ cells from naïve mice tested in parallel were negative. G Mice were primed with 5 × 1010vp of AdC6-gDPolN. h Mice were prime with 5 × 1010vp of AdC6-gDPolN and then boosted 8 weeks later with 5 × 1010vp of AdC7-gDPolN. g, h Pooled splenocyts were tested 6 weeks after completion of vaccination by ICS for IFN-γ production in response to individual peptides representing the PolN sequence. Data are shown as frequencies over CD44+CD8+ cells. The dotted line at 0.1% reflects the experimental cut-off for positive samples. CD44+CD8+ cells from naïve mice tested in parallel were negative

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