Fig. 3

Systemic and mucosal antibody responses against CDV. The mice were immunized with soluble CDV H and nanoparticles, and sera and nasal wash samples were collected 2 weeks after each immunization. The final samples of tested data are shown. ELISA plates were coated with soluble CDV H protein, and the samples were diluted 2 × stepwise. Bound antibody was detected by binding HRP-conjugated goat anti-mouse IgG, IgG1, IgG2a, and IgA. A, serum IgG titers; B, IgG isotypes; C, IgA titers of nasal wash; D, CDV NA titers. Assays were performed as described in materials and methods. Results are expressed as means ± standard deviations. P < 0.05 was considered statistically significant.**P < 0.01, * P < 0.05