Table 1 Primer sequences used real-time PCR
From: Human rhinovirus serotypes induces different immune responses
Genes | Primer sequences |
|---|---|
ICAM-1 | Forward 5′-GCAGACAGTGACCATCTACAGCTT-3′ Reverse 5′-CTTCTGAGACCTCTGGCTTCGT-3′ |
LDL-R | Forward 5′-GACGTGGCGTGAACATCTC-3′ Reverse 5′-CTGGCAGGCAATGCTTTGG-3′ |
TLR3 | Forward 5′-TTTGCGAAGAGGAATGTTTAAATCT-3′ Reverse 5′-CACCTATCCGTTCTTTCTGAACTG-3′ |
RIG-1 | Forward 5′-GCTGATGAAGGCATTGACATTG-3′ Reverse 5′-CAGCATTACTAGTCAGAAGGAAGCA-3′ |
MDA-5 | Forward 5′-CCCATGACACAGAATGAACAAAA-3′ Reverse 5′-CGAGACCATAACGGATAACAATGT-3′ |
NK-κB | Forward 5′-CCATGACAGCAAATCTCC-3′ Reverse 5′- TAAACTTCATCTCCACCCC-3′ |
IRF3 | Forward 5′-TCTTCCAGCAGACCATCTCC-3′ reverse 5′-TGCCTCACGTAGCTCATCAC-3′ |
IRF7 | Forward 5′-TACCATCTACCTGGGCTTCG-3′ Reverse 5′-TGCTGCTATCCAGGGAAGAC-3′ |
STAT1 | Forward 5′-ATCAGGCTCAGTCGGGGAAT-3′ Reverse 5′-TGGTCTCGTGTTCTCTGTTCTGC-3′ |
STAT2 | Forward 5′-GCCCTAGTTCCAGCTCTAATG-3′ Reverse 5′- CAGGCTCATTGTGGTCTCTAAT-3′ |
IRF9 | Forward 5′-CCCGAAAACTCCGGAACTG-3′ Reverse 5′-CAGCACACTCCGGGAAACT-3′ |
GAPDH | Forward 5′-GACCCCTTCATTGACCTC-3′ Reverse 5′-GCTAAGCAGTTGGTGGTG-3′ |