Table 2 Comparison of RNAscope and PCR results in patient cervical scraped cells (a) RNAscope positive whereas PCR negative. (b) RNAscope negative whereas PCR positive. (c) RNAscope and PCR both positive whereas inconsistence between high-risk and low-risk subtypes
Patient # | RNAscope results | qPCR Analysis of HPV DNA | ||||
|---|---|---|---|---|---|---|
Probe V-HPV-HR18 | Probe V-HPV-LR6 | Probe Hs-TERC | HPV subtype | Patient age | Sample collector | |
(a) | ||||||
P#21 |  +  | − | − | − | 45 | Doctor #2 |
|  | Nucleus, ≥ 3 cells |  |  | Postsurgery of Loop Eelectrosurgical Excision Procedure (LEEP) |  |  |
P#67 |  +  | − | − | − | 38 | Doctor #4 |
|  | Nucleus and cluster, ≥ 3 cells |  |  | CINI Cervical Intraepithelial Neoplasia |  |  |
P#32 |  +  |  +  | − | − | 53 | Doctor #1 |
|  | Nucleus, < 3 cells | Cluster, < 3 cells |  |  |  |  |
P#23 | − |  +  | − | − | 49 | Doctor #2 |
|  |  | Nucleus, ≥ 3 cells |  |  |  |  |
P#59 |  +  |  +  | − | − | 32 | Doctor #4 |
|  | Cluster, ≥ 3 cells | Cluster, ≥ 3 cells |  | CINII, postsurgery of Loop Eelectrosurgical Excision Procedure(LEEP) 2 years |  |  |
P#2 |  +  |  +  | − | − | 34 | Doctor #1 |
|  | Cluster, < 3 cells | Cluster, > 3 cells |  | Postsurgery of Loop Eelectrosurgical Excision Procedure (LEEP) |  |  |
P#80 |  +  | − | − | − | 28 | Doctor #1 |
|  | Cluster, < 3 cells |  |  |  |  |  |
P#18 | − |  +  | − | − | 57 | Doctor #1 |
|  |  | Cluster, < 3 cells |  |  |  |  |
(b) | ||||||
P#61 | − | − |  +  | 52 | 60 | Doctor #4 |
|  |  |  | Nucleus, > 10 cells | CINI−II, postsurgery of Leep (3 years) |  |  |
P#10 | − | − | − | 51, 52 | 34 | Doctor #1 |
P#15 | − | − | − | 39 | 36 | Doctor #1 |
P#17 | − | − | − | 58 | 27 | Doctor #1 |
| Â | Â | Â | Â | CKC after | Â | Â |
P#27 | − | − | − | 33, 52, 58 | 64 | Doctor #3 |
P#54 | − | − | − | 33 | 32 | Doctor #4 |
P#62 | − | − | − | 81 | 53 | Doctor #4 |
| Â | Â | Â | Â | CINI | Â | Â |
P#100 | − | − | − | 44 | 45 | Doctor #4 |
P#96 | − | − | − | 16 | 71 | Doctor #1 |
| Â | Â | Â | Â | Vaginal Intraepithelial Neoplasia (VaIN) III, | Â | Â |
(c) | ||||||
P#26 |  +  | − | − | 44, 45 | 39 | Doctor #1 |
|  | Cyto, > 10 cells |  |  | CINIII |  |  |
P#48 |  +  |  +  |  +  | 52 | 60 | Doctor #1 |
|  | Cyto, > 10 cells | Cluster, > 10 cells | Nucleus, > 10 cells |  |  |  |
P#4 |  +  |  +  | − | 53, 56 | 53 | Doctor #1 |
|  | Nuclear, ≥ 3 cells | Nuclear, ≥ 3 cells |  | Postsurgery of Cold Knife Conization (CKC) |  |  |
P#9 |  +  |  +  | − | 43 | 69 | Doctor #1 |
|  | Cluster, < 3 cells | Cluster, ≥ 3 cells |  |  |  |  |
P#33 |  +  |  +  | − | 52 | 37 | Doctor #1 |
|  | Cluster, < 3 cells | Cluster, < 3 cells |  | HPV infection history, after laser CO2 vaporization therapy |  |  |
P#73 |  +  | − | − | 42, 43 | 42 | Doctor #1 |
|  | Cluster, < 3 cells |  |  |  |  |  |
P#5 | − |  +  | − | 11, 59 | 62 | Doctor #1 |
|  |  | Cyto, > 10 cells |  |  |  |  |
P#53 | − |  +  | − | 45 | 35 | Doctor #4 |
|  |  | Cyto, < 3 cells |  |  |  |  |
P#31 | − |  +  | − | 56 | 56 | Doctor #1 |
|  |  | Cluster, > 10 cells |  |  |  |  |
P#6 | − |  +  | − | 68, 43 | 27 | Doctor #1 |
|  |  | Cluster, < 3 cells |  | Postsurgery of Cold Knife Conization (CKC) |  |  |
P#19 | − |  +  | − | 58 | 37 | Doctor #1 |
|  |  | Cluster, < 3 cells |  |  |  |  |
P#30 | − |  +  | − | 16, 68, 42 | 30 | Doctor #1 |
|  |  | Cluster, < 3 cells |  |  |  |  |