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Table 2 Comparison of RNAscope and PCR results in patient cervical scraped cells (a) RNAscope positive whereas PCR negative. (b) RNAscope negative whereas PCR positive. (c) RNAscope and PCR both positive whereas inconsistence between high-risk and low-risk subtypes

From: Human papillomavirus E6E7 mRNA and TERC lncRNA in situ detection in cervical scraped cells and cervical disease progression assessment

Patient # RNAscope results qPCR Analysis of HPV DNA
Probe V-HPV-HR18 Probe V-HPV-LR6 Probe Hs-TERC HPV subtype Patient age Sample collector
(a)
P#21  +  − − − 45 Doctor #2
  Nucleus, ≥ 3 cells    Postsurgery of Loop Eelectrosurgical Excision Procedure (LEEP)   
P#67  +  − − − 38 Doctor #4
  Nucleus and cluster, ≥ 3 cells    CINI Cervical Intraepithelial Neoplasia   
P#32  +   +  − − 53 Doctor #1
  Nucleus, < 3 cells Cluster, < 3 cells     
P#23 −  +  − − 49 Doctor #2
   Nucleus, ≥ 3 cells     
P#59  +   +  − − 32 Doctor #4
  Cluster, ≥ 3 cells Cluster, ≥ 3 cells   CINII, postsurgery of Loop Eelectrosurgical Excision Procedure(LEEP) 2 years   
P#2  +   +  − − 34 Doctor #1
  Cluster, < 3 cells Cluster, > 3 cells   Postsurgery of Loop Eelectrosurgical Excision Procedure (LEEP)   
P#80  +  − − − 28 Doctor #1
  Cluster, < 3 cells      
P#18 −  +  − − 57 Doctor #1
   Cluster, < 3 cells     
(b)
P#61 − −  +  52 60 Doctor #4
    Nucleus, > 10 cells CINI−II, postsurgery of Leep (3 years)   
P#10 − − − 51, 52 34 Doctor #1
P#15 − − − 39 36 Doctor #1
P#17 − − − 58 27 Doctor #1
     CKC after   
P#27 − − − 33, 52, 58 64 Doctor #3
P#54 − − − 33 32 Doctor #4
P#62 − − − 81 53 Doctor #4
     CINI   
P#100 − − − 44 45 Doctor #4
P#96 − − − 16 71 Doctor #1
     Vaginal Intraepithelial Neoplasia (VaIN) III,   
(c)
P#26  +  − − 44, 45 39 Doctor #1
  Cyto, > 10 cells    CINIII   
P#48  +   +   +  52 60 Doctor #1
  Cyto, > 10 cells Cluster, > 10 cells Nucleus, > 10 cells    
P#4  +   +  − 53, 56 53 Doctor #1
  Nuclear, ≥ 3 cells Nuclear, ≥ 3 cells   Postsurgery of Cold Knife Conization (CKC)   
P#9  +   +  − 43 69 Doctor #1
  Cluster, < 3 cells Cluster, ≥ 3 cells     
P#33  +   +  − 52 37 Doctor #1
  Cluster, < 3 cells Cluster, < 3 cells   HPV infection history, after laser CO2 vaporization therapy   
P#73  +  − − 42, 43 42 Doctor #1
  Cluster, < 3 cells      
P#5 −  +  − 11, 59 62 Doctor #1
   Cyto, > 10 cells     
P#53 −  +  − 45 35 Doctor #4
   Cyto, < 3 cells     
P#31 −  +  − 56 56 Doctor #1
   Cluster, > 10 cells     
P#6 −  +  − 68, 43 27 Doctor #1
   Cluster, < 3 cells   Postsurgery of Cold Knife Conization (CKC)   
P#19 −  +  − 58 37 Doctor #1
   Cluster, < 3 cells     
P#30 −  +  − 16, 68, 42 30 Doctor #1
   Cluster, < 3 cells     
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Virology Journal

ISSN: 1743-422X