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Table 2 Comparison of RNAscope and PCR results in patient cervical scraped cells (a) RNAscope positive whereas PCR negative. (b) RNAscope negative whereas PCR positive. (c) RNAscope and PCR both positive whereas inconsistence between high-risk and low-risk subtypes

From: Human papillomavirus E6E7 mRNA and TERC lncRNA in situ detection in cervical scraped cells and cervical disease progression assessment

Patient #

RNAscope results

qPCR Analysis of HPV DNA

Probe V-HPV-HR18

Probe V-HPV-LR6

Probe Hs-TERC

HPV subtype

Patient age

Sample collector

(a)

P#21

 + 

45

Doctor #2

 

Nucleus, ≥ 3 cells

  

Postsurgery of Loop Eelectrosurgical Excision Procedure (LEEP)

  

P#67

 + 

38

Doctor #4

 

Nucleus and cluster, ≥ 3 cells

  

CINI Cervical Intraepithelial Neoplasia

  

P#32

 + 

 + 

53

Doctor #1

 

Nucleus, < 3 cells

Cluster, < 3 cells

    

P#23

 + 

49

Doctor #2

  

Nucleus, ≥ 3 cells

    

P#59

 + 

 + 

32

Doctor #4

 

Cluster, ≥ 3 cells

Cluster, ≥ 3 cells

 

CINII, postsurgery of Loop Eelectrosurgical Excision Procedure(LEEP) 2 years

  

P#2

 + 

 + 

34

Doctor #1

 

Cluster, < 3 cells

Cluster, > 3 cells

 

Postsurgery of Loop Eelectrosurgical Excision Procedure (LEEP)

  

P#80

 + 

28

Doctor #1

 

Cluster, < 3 cells

     

P#18

 + 

57

Doctor #1

  

Cluster, < 3 cells

    

(b)

P#61

 + 

52

60

Doctor #4

   

Nucleus, > 10 cells

CINI−II, postsurgery of Leep (3 years)

  

P#10

51, 52

34

Doctor #1

P#15

39

36

Doctor #1

P#17

58

27

Doctor #1

    

CKC after

  

P#27

33, 52, 58

64

Doctor #3

P#54

33

32

Doctor #4

P#62

81

53

Doctor #4

    

CINI

  

P#100

44

45

Doctor #4

P#96

16

71

Doctor #1

    

Vaginal Intraepithelial Neoplasia (VaIN) III,

  

(c)

P#26

 + 

44, 45

39

Doctor #1

 

Cyto, > 10 cells

  

CINIII

  

P#48

 + 

 + 

 + 

52

60

Doctor #1

 

Cyto, > 10 cells

Cluster, > 10 cells

Nucleus, > 10 cells

   

P#4

 + 

 + 

53, 56

53

Doctor #1

 

Nuclear, ≥ 3 cells

Nuclear, ≥ 3 cells

 

Postsurgery of Cold Knife Conization (CKC)

  

P#9

 + 

 + 

43

69

Doctor #1

 

Cluster, < 3 cells

Cluster, ≥ 3 cells

    

P#33

 + 

 + 

52

37

Doctor #1

 

Cluster, < 3 cells

Cluster, < 3 cells

 

HPV infection history, after laser CO2 vaporization therapy

  

P#73

 + 

42, 43

42

Doctor #1

 

Cluster, < 3 cells

     

P#5

 + 

11, 59

62

Doctor #1

  

Cyto, > 10 cells

    

P#53

 + 

45

35

Doctor #4

  

Cyto, < 3 cells

    

P#31

 + 

56

56

Doctor #1

  

Cluster, > 10 cells

    

P#6

 + 

68, 43

27

Doctor #1

  

Cluster, < 3 cells

 

Postsurgery of Cold Knife Conization (CKC)

  

P#19

 + 

58

37

Doctor #1

  

Cluster, < 3 cells

    

P#30

 + 

16, 68, 42

30

Doctor #1

  

Cluster, < 3 cells