Table 1 Comparison of RNAscope and PCR results in patient cervical scraped cells. The detection results are consistent between the two methods
Patient # | RNAscope results | qPCR Analysis of HPV DNA | ||||
|---|---|---|---|---|---|---|
Probe V-HPV-HR18 | Probe V-HPV-LR6 | Probe Hs-TERC | HPV subtype | Patient age | Sample collector | |
P#1 |  +  | − | − | 16, 53, 56, 58 | 39 | Doctor #1 |
|  | Cyto and nucleus, > 10 cells |  |  | Atypical squamous cells of undetermined significance (ASCUS) |  |  |
P#20 |  +  | − |  +  | 16 | 42 | Doctor #1 |
|  | Cyto, > 10 cells |  |  | Cervical Intraepithelial Neoplasia (CIN) II–III |  |  |
P#22 |  +  | − | − | 52 | 47 | Doctor #1 |
|  | Cyto and nucleus, ≥ 3 cells |  |  |  |  |  |
P#24 |  +  | − | − | 58 | 30 | Doctor #2 |
|  | Cyto and nucleus, ≥ 3 cells |  |  | Postsurgery of Cold knife cone (CKC) |  |  |
P#28 |  +  | − | − | 51 | 32 | Doctor #1 |
|  | Cyto and nucleus, ≥ 3 cells |  |  | LSIL |  |  |
P#40 |  +  | − |  +  | Not test | 63 | Doctor #1 |
|  | Cyto, ≥ 3 cells |  |  | Early invasion in cervical carcinoma |  |  |
P#41 |  +  | − | − | 52 | 47 | Doctor #3 |
|  | Cyto and nucleus, ≥ 3 cells |  |  | Postsurgery of Loop Eelectrosurgical Excision Procedure (LEEP) |  |  |
P#47 |  +  | − | − | 18 | 28 | Doctor #1 |
|  | Cyto, ≥ 3 cells |  |  |  |  |  |
P#60 |  +  | − |  +  | 53 | 25 | Doctor #2 |
|  | Cyto, ≥ 3 cells |  |  | HPV infection history |  |  |
P#69 |  +  | − | − | 52 | 23 | Doctor #4 |
|  | Cyto and nucleus, ≥ 3 cells |  |  |  |  |  |
P#77 |  +  | − |  +  | 82, 42 | 42 | Doctor #1 |
|  | Nuclear, ≥ 3 cells |  |  |  |  |  |
P#82 |  +  | − | − | 6, 59 | 58 | Doctor #2 |
|  | Cyto and nucleus, ≥ 3 cells |  |  |  |  |  |
P#83 |  +  | − | − | 53 | 65 | Doctor #4 |
|  | Cyto and nucleus, ≥ 3 cells |  |  | CINI |  |  |
P#8 |  +  | − | − | 52, 53, 58 | 32 | Doctor #1 |
|  | Cyto, < 3 cells |  |  |  |  |  |
P#39 |  +  | − | − | 16, 18 | 37 | Doctor #1 |
|  | Cyto, < 3 cells |  |  | CINI |  |  |
P#49 |  +  | − | − | 51 | 32 | Doctor #1 |
|  | Cluster, ≥ 3 cells |  |  | Postsurgery of LEEP 2 years |  |  |
P#98 |  +  | − | − | 56 | 61 | Doctor #2 |
|  | Cluster, ≥ 3 cells |  |  |  |  |  |
P#57 |  +  | − | − | 53 | 29 | Doctor #2 |
|  | Cluster, < 3 cells |  |  | HPV infection history and CINI |  |  |
P#71 |  +  | − | − | 52 | 45 | Doctor #4 |
|  | Cluster, < 3 cells |  |  | CINI |  |  |
P#75 |  +  | − |  +  | 52 | 50 | Doctor #4 |
|  | Cluster, < 3 cells |  |  | CINII |  |  |
P#81 |  +  | − | − | 33 | 50 | Doctor #1 |
|  | Cluster, < 3 cells |  |  |  |  |  |
P#89 |  +  |  +  | − | 6, 51 | 24 | Doctor #1 |
|  | Cluster, < 3 cells | Cluster, < 3 cells |  |  |  |  |
P#71 |  +  | − | − | 52 | 45 | Doctor #4 |
|  | Cluster, < 3 cells |  |  | CINI |  |  |