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Table 1 Comparison of RNAscope and PCR results in patient cervical scraped cells. The detection results are consistent between the two methods

From: Human papillomavirus E6E7 mRNA and TERC lncRNA in situ detection in cervical scraped cells and cervical disease progression assessment

Patient # RNAscope results qPCR Analysis of HPV DNA
Probe V-HPV-HR18 Probe V-HPV-LR6 Probe Hs-TERC HPV subtype Patient age Sample collector
P#1  +  16, 53, 56, 58 39 Doctor #1
  Cyto and nucleus, > 10 cells    Atypical squamous cells of undetermined significance (ASCUS)   
P#20  +   +  16 42 Doctor #1
  Cyto, > 10 cells    Cervical Intraepithelial Neoplasia (CIN) II–III   
P#22  +  52 47 Doctor #1
  Cyto and nucleus, ≥ 3 cells      
P#24  +  58 30 Doctor #2
  Cyto and nucleus, ≥ 3 cells    Postsurgery of Cold knife cone (CKC)   
P#28  +  51 32 Doctor #1
  Cyto and nucleus, ≥ 3 cells    LSIL   
P#40  +   +  Not test 63 Doctor #1
  Cyto, ≥ 3 cells    Early invasion in cervical carcinoma   
P#41  +  52 47 Doctor #3
  Cyto and nucleus, ≥ 3 cells    Postsurgery of Loop Eelectrosurgical Excision Procedure (LEEP)   
P#47  +  18 28 Doctor #1
  Cyto, ≥ 3 cells      
P#60  +   +  53 25 Doctor #2
  Cyto, ≥ 3 cells    HPV infection history   
P#69  +  52 23 Doctor #4
  Cyto and nucleus, ≥ 3 cells      
P#77  +   +  82, 42 42 Doctor #1
  Nuclear, ≥ 3 cells      
P#82  +  6, 59 58 Doctor #2
  Cyto and nucleus, ≥ 3 cells      
P#83  +  53 65 Doctor #4
  Cyto and nucleus, ≥ 3 cells    CINI   
P#8  +  52, 53, 58 32 Doctor #1
  Cyto, < 3 cells      
P#39  +  16, 18 37 Doctor #1
  Cyto, < 3 cells    CINI   
P#49  +  51 32 Doctor #1
  Cluster, ≥ 3 cells    Postsurgery of LEEP 2 years   
P#98  +  56 61 Doctor #2
  Cluster, ≥ 3 cells      
P#57  +  53 29 Doctor #2
  Cluster, < 3 cells    HPV infection history and CINI   
P#71  +  52 45 Doctor #4
  Cluster, < 3 cells    CINI   
P#75  +   +  52 50 Doctor #4
  Cluster, < 3 cells    CINII   
P#81  +  33 50 Doctor #1
  Cluster, < 3 cells      
P#89  +   +  6, 51 24 Doctor #1
  Cluster, < 3 cells Cluster, < 3 cells     
P#71  +  52 45 Doctor #4
  Cluster, < 3 cells    CINI   
  1. P patient