Molecular detection, phylogenetic analysis and genetic diversity of recently isolated foot-and-mouth disease virus serotype A African topotype, Genotype IV

Table 1 Primers used in real-time RT-PCR of 3D gene, one-step RT-PCR and sequencing reaction of 1D gene of FMDV

Serotype	Primer designation	Primer sequence (5'-3')	Amplicon size	References
All serotypes	3D-F	ACTGGGTTTTACAAACCTGTGA	106 bp	[20]
	3D-R	GCGAGTCCTGCCACGGA
	3D-Probe	TCCTTTGCACGCCGTGGGAC
O	O-Egy-F	CCTCCTTCAAYTACGGT	283 bp	[21]
A	A–1C612F	TAGCGCCGGCAAAGACTTTGA	814 bp	[14]
SAT2	SAT2-Egy-F	TGAYCGCAGTACACAYGTYC	666 bp	[22]
A,O, and SAT2	EUR–2B52R	GACATGTCCTCCTGCATCTGGTTGAT	–	[14]
All serotypes (pan-FMDV)	FMD-3161-F	TCG CVC AGT ACT ACR CAC AGT A	1143 bp	[23]
All serotypes (pan-FMDV)	FMD-4303-R	TGA CGT CRG AGA AGA AGA ARG G	1143 bp	[23]