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Table 3 The reproducibility of the PeV-A real-time RT- PCR

From: Development of a real-time RT-PCR assay for the detection of pan-human parechoviruses

Copies/reaction

No. of positive samples/no. of samples test by real-time

RT-PCR for detection of PeV-A1 and PeV-A3a

PeV-A1

PeV-A3

107 copies/μL

8/8

8/8

106 copies/μL

8/8

8/8

105 copies/μL

8/8

8/8

104 copies/μL

8/8

8/8

103 copies/μL

8/8

8/8

102 copies/μL

8/8

8/8

101 copies/μL

0/8

0/8

  1. aThe results were interpreted as ‘positive’ for threshold cycle (Ct) values ≤ 35 and ‘negative’ for Ct > 35