Table 2 Forward (F) and reverse (R) primers and probe (P) for the detection of human parechovirus (PeV-A) and primers for amplification of the VP1 region of PeV-A
From: Development of a real-time RT-PCR assay for the detection of pan-human parechoviruses
Name | Primer sequence (5′–3′) | Nucleotide Position (nt)* | References |
|---|---|---|---|
PeV-A-5’UTR-F | TGACGTGTGGGAACTTRTTGG | 352–372 | This study |
PeV-A-5’UTR-R | CCTTCGTGGGCCYTACAACTAGTG | 538–561 | This study |
PeV-A-5’UTR-P | CYCTGGGGCCAAAAGCCAAGGTTT | 433–456 | This study |
PeV-A-VP1-F1 | TTYTCYACTTGGATGMGGAARA | 2159–2180 | This study |
PeV-A-VP1-R1 | ATRCTYGACATHARNCCWGC | 3392–3411 | This study |
PeV-A-VP1-F2 | CNTGGGGYTCVCARATGGAYYT | 2340–2361 | This study |
PeV-A-VP1-R2 | CCATARTGYTTRTARAAVCCYCTRT | 3081–3105 | This study |