Fig. 5

Neutralization of IL-38 regulated Th1 and Th17 cells differentiation in vivo. A Representative flow cytometry dot plots of splenic CD4⁺IFN-γ⁺ Th1 cells and CD4⁺IL-17⁺ Th17 cells in the Sham, PBS, IL-38N, and IgG groups. Numbers in upper left quadrants and lower right quadrants indicate the separate percentages of CD4⁺ Th1 cells and CD4⁺ Th17 cells. B Abundance of Th1 cells in different groups. C Cardiac mRNA expression levels of T-bet in different groups. D Abundance of Th17 cells in different groups. E Cardiac mRNA expression levels of RORγt in different groups. ^**P < 0.01, compared to Sham group; ^#P < 0.05, ^##P < 0.01, compared to IgG group. Data are expressed as mean ± SD