From: Porcine reproductive and respiratory syndrome virus genetic variability a management and diagnostic dilemma
Samples
RT-qPCR
Sanger sequencing ORF5 (% similarity)
Vaccine-like preferential RT-qPCR
Difference in ct value of preferential RT-qPCR and RT-qPCR
1
27.8
94.9
+ (ct 33.4)
5.6
2
26.7
97.3
−
N/A
3
22.7
97.7
+ (ct 26.9)
4.2