Fig. 8

Intranasal vaccination with γ-Flu-CCL21-Poly (I:C) elevates protective responses at a high viral challenge dose. One week following the third immunization, mice were infected intranasally with 10 LD50 of A/PR/8/34 (H1N1) and monitored for 14 days to determine survival changes. A loss of 25% of total body weight was used as an endpoint. Survival data are presented as the percentages of animals surviving among the total number monitored (a). On day 4 post-challenge, three mice in each group were sacrificed, and lung viral loads were determined by MDCK/HA assay (b). ** Indicates statistically significant difference between the with γ-Flu-CCL21-Poly (I:C) and γ-Flu group (p < 0.01), as calculated by one-way ANOVA. The graph also shows a statistically significant difference between all vaccinated and control groups (p < 0.001) (b)