Fig. 2

Effects of CCL21 and Poly (I:C) as adjuvants on specific IgG antibody levels. Columns represent relative antibody expression in each treated group (n = 3). The measured values for influenza-specific total IgG (a), IgG1 (b), and IgG2a (c) in immune sera were reported as the mean ± SD, and One way ANOVA is used to calculate the statistical significance. Differences between all treatment groups and control groups were statistically high significant (***p < 0.001). The highest level of total IgG and IgG1 isotype was also observed in mice primed with γ-Flu plus CCL21-Poly (I:C) while there was no significant difference among the groups immunized with γ-Flu-CCL21, γ-Flu-Poly (I:C), or γ-Flu-CCL21-Poly (I:C) in the number of IgG2a secreting cells