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Fig. 3 | Virology Journal

Fig. 3

From: GATA binding protein 4 promotes the expression and transcription of hepatitis B virus by facilitating hepatocyte nuclear factor 4 alpha in vitro

Fig. 3

siGATA4 negatively regulates HBV. HepG2 cells were transfected with siGATA4 (10 nM) for 24 h, and then transfected with HBV cccDNA (500 ng/mL) for 48 h. HepG2.2.15 cells carry the complete genome of HBV, so these were only transfected with siGATA4 for 48 h. a Relative mRNA of GATA4 in cells detected via RT-qPCR, β-actin was used as the internal control. b Analysis of HBV DNA copies in cells determined by qPCR. c HBsAg levels in the culture medium detected through enzyme-linked immunosorbent assays. d Relative levels of HBV 3.5 kb pgRNA and total RNA in cells analyzed via RT-qPCR, β-actin was used as the internal control. e Protein of GATA4 (51 kDa) analyzed via WB. All statistics are marked as NS P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001

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Virology Journal

ISSN: 1743-422X