Genomic and functional characterization of five novel Salmonella-targeting bacteriophages

Table 2 Sequence variation between phages UPWr_S2, UPWr_S3 and UPWr_S4

Gene product	Protein function	UPWr_S2 vs UPWr_S3	UPWr_S2 vs UPWr_S4	UPWr_S3 vs UPWr_S4
gp16	Putative head decoration protein	–	R132G^*	R132G
gp20	Putative major capsid protein	–	V338A	V338A
Region between gp20-21		–	Substitution of g to a	–
gp25	Putative protein	R185W	R185W	–
gp30	Putative tail protein	A94V	–	V94A
Region between gp30-gp31		Deletion of 1 t^** in UPWr_S2	Deletion of 1 t in UPWr_S2	–
gp42	Tail fiber protein	Deletion of 6 AA in UPWr_S2 374–379 (MYKDNG)	Deletion of 6 AA in UPWr_S2 374–379 (MYKDNG)	–
gp43	Tailspike	–	S2P, G464D	S2P, G464D

Whole genome alignment revealed the presence of a few differences in nucleotide sequences between UPWr_S2, UPWr_S3 and UPWr_S4 phages. These differences included deletions and substitutions resulting in amino acid alterations or single nucleotide changes in intergenic regions. There are 4 common substitutions between these phages located in predicted head decoration (gp16), putative major capsid protein (gp20) and tailspike protein with endorhamnosidase function (gp43)
^*Letters corresponding to amino acids are written with capital letters
^**Letters corresponding to nucleotides are lower case and italicized
– no changes

ISSN: 1743-422X