Genomic and functional characterization of five novel Salmonella-targeting bacteriophages

Table 1 UPWr_S phages’ morphological features, genome size and biological characteristics

	UPWr_S1	UPWr_S2	UPWr_S3	UPWr_S4	UPWr_S5
Capsid size, nm ± SE^*	52 ± 6	57 ± 4	52 ± 5	56 ± 4.5	51 ± 4
Tail size, nm ± SE	134 ± 2	136 ± 7.1	138 ± 5	129 ± 4	131 ± 3.6
Genome size, bp	44 417	44 225	44 154	44 330	44 548
GC content, %	49.85	50.02	50.01	50.02	49.99
MOI	1	0.1	0.1	1	1
Latent period, min	15	12	9	24	26
Burst size, PFU/cell	201	89	92	48	23
Adsorption degree^**	90	93	99	80	92

UPWr_S phages’ morphological and biological characterization revealed their high degree of similarity. Calculated latent period and burst size for each phage showed the shorter latent period with a higher burst size and lower MOI of phages UPWr_S2 and UPWr_S3
^*Head diameter is calculated for isometric capsids. All measurements were made with the program ImageJ. 35 particles were measured for each phage and standard deviation was calculated (± SD)
^**Phage particles adsorbed within 10 min

ISSN: 1743-422X