Table 4 Comparison of three qRT-PCR assays for the detection of HeV-g2, HeV N assay, HeV-g2 M assay and HeV-g2 N assay using bat samples from 2013 to 2021; values shown are Ct values
From: A new Hendra virus genotype found in Australian flying foxes
Sample ID-sample type | HeV N assay | HeV-g2 M assay | HeV-g2 N assay |
|---|---|---|---|
2013–01-kidney*# | 31.3 | 28.8 | NT** |
2013–01-spleen | 40.9 | 33.4 | NT |
2015–01-spleen | Negative | 38.4 | Negative |
2019–01-kidney | Negative | Negative | Negative |
2019–01-spleen | Negative | 34.2 | NT |
2019–02-kidney | Negative | Negative | Negative |
2019–02-spleen | Negative | 29.6 | 31.8 |
2020–01-kidney | 43.2 | 30.6 | 28.3 |
2020–01-spleen# | 34.8 | 30.6 | 32.3 |
2020–02-kidney | Neg | 34.9 | 35.5 |
2020–02-spleen# | 34.4 | 23.1 | 23.0 |
2020–03-kidney# | 37.1 | 27.4 | 26.6 |
2020–03-spleen | Negative | Negative | Negative |
2021–01-kidney | Negative | Neg | 41.8 |
2021–01-spleen | Negative | 33.2 | 40.3 |
2021–02-kidney | Negative | Negative | Negative |
2021–02-spleen | Negative | 37.0 | 41.3 |
2021–03-kidney | Negative | Negative | Negative |
2021–03-spleen | Negative | 36.9 | 40.2 |
2021–04-kidney | 39.0 | 31.1 | 31.4 |
2021–04-spleen | Negative | Negative | 36.8 |
Total no. of positive | 7/21 (14%) | 14/21 (67%) | 12/18(67%) |