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Table 4 Comparison of three qRT-PCR assays for the detection of HeV-g2, HeV N assay, HeV-g2 M assay and HeV-g2 N assay using bat samples from 2013 to 2021; values shown are Ct values

From: A new Hendra virus genotype found in Australian flying foxes

Sample ID-sample type HeV N assay HeV-g2 M assay HeV-g2 N assay
2013–01-kidney*# 31.3 28.8 NT**
2013–01-spleen 40.9 33.4 NT
2015–01-spleen Negative 38.4 Negative
2019–01-kidney Negative Negative Negative
2019–01-spleen Negative 34.2 NT
2019–02-kidney Negative Negative Negative
2019–02-spleen Negative 29.6 31.8
2020–01-kidney 43.2 30.6 28.3
2020–01-spleen# 34.8 30.6 32.3
2020–02-kidney Neg 34.9 35.5
2020–02-spleen# 34.4 23.1 23.0
2020–03-kidney# 37.1 27.4 26.6
2020–03-spleen Negative Negative Negative
2021–01-kidney Negative Neg 41.8
2021–01-spleen Negative 33.2 40.3
2021–02-kidney Negative Negative Negative
2021–02-spleen Negative 37.0 41.3
2021–03-kidney Negative Negative Negative
2021–03-spleen Negative 36.9 40.2
2021–04-kidney 39.0 31.1 31.4
2021–04-spleen Negative Negative 36.8
Total no. of positive 7/21 (14%) 14/21 (67%) 12/18(67%)
  1. *Virus isolation was attempted for samples in bold
  2. **NT not tested
  3. #NGS was conducted on these samples