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Table 1 Primers and probes for qRT-PCR and conventional PCR

From: A new Hendra virus genotype found in Australian flying foxes

qRT-PCR
HeV-g2 M gene
Forward primer HeV-g2-M-F: 5’-CTGATCTACGTTACGGCAAACCTT-3’
Reverse primer HeV-g2-M-R: 5’-GGCCCGCTTCACCATCTCTTAC-3’
Probe HeV-g2-M-P: 5’-FAM-CAGCATTGAATATTGACCCGCCAGTCA-BHQ1-3’
HeV-g2 N gene
Forward primer HeV-g2-N-F: 5’-TGCGACAGATCCCAGTAGTATTAAAT-3’
Reverse primer HeV-g2-N-R: 5’-GGCAGCTTATTCGGCAAAAG-3’
Probe HeV-g2-N-P: 5’-FAM-CTCTGGTGACGGAACACAAATGCAAATTTC-BHQ1-3’
Conventional PCR   
Primary RT-PCR   
Primer name Primer sequences PCR product
Forward primer HeV-M-5481F 5’- GCCCGCTTCATCATCTCTT-3’ 300 bp
Reverse primer HeV-M-5871R1: 5’CCACTTTGGTTCCGTCTTTG-3’
Semi-nested PCR
Forward primer HeV-M-5481F 5’- GCCCGCTTCATCATCTCTT-3’ 210 bp
Reverse primer HeV-M-5691R2: 5’-GCAATAGCGTTGTTCCTTCTG-3’