Fig. 3
From: Genome-wide identification of endogenous viral sequences in alfalfa (Medicago sativa L.)
Confirmation of the randomly selected EVEs by PCR. M, 1Â Kb Plus DNA Ladder (Thermo Fisher Scientific, USA); Lane 1: a 293Â bp PCR product amplified using alfalfa DNA and primers pair LN968/969 designed for the FMV-like insert in Msat4n/chr1.1. Lane 2: amplification from P. sativum DNA using primers pair LN968/969. Lane 3: a 276Â bp PCR product amplified from alfalfa DNA using primers pair LN970/971 for the FMV-like insert located in Msativa4n/chr2.2. Lane 4: Amplification from P. sativum DNA using primers pair LN970/971. Lane 5: a 219Â bp PCR product amplified using alfalfa DNA and primers pair LN978/979 for the FMV-like insert in Msat/chr3.2. Lane 6: Amplification from P. sativum DNA with primers pair LN978/979. Lane 7: a 296Â bp PCR product amplified using alfalfa DNA and primers pair LN980/981 for the FMV-like insert located in Msat/chr5.1. Lane 8: amplification from P. sativum DNA using primers pair LN980/981