Table 4 RT-qPCR-based relative expression (log2 fold change, LFC) of transcripts and CBSV concentration in RNAi-silenced/control Kaleso plants at different time points of agro-inoculation relative to 0 daai
Sample type | LFC of PAL1 in RNAi-silenced plants at 20–48 days post agro-infiltration: | LFC of CBSV abundance at 14–28 days after inoculation of treated plants: | |||||
|---|---|---|---|---|---|---|---|
20 | 34 | 41 | 48 | 34 | 41 | 48 | |
Non-silenced, absolute control | 0.5 | 3.7 | 2.0 | 4.8 | ND | ND | ND |
− 0.2 | − 0.7 | 2.0 | 3.8 | ND | ND | ND | |
Non-silenced, control with empty vector | − 0.3 | 1.3 | − 3.3 | − 0.7 | ND | ND | ND |
− 1.0 | − 0.7 | − 3.3 | − 9.0 | ND | ND | ND | |
CBSV inoculation control, susceptible Kalawe | 0.6 | − 3.3 | − 0.5 | 1.8 | 0.0 | 3.7 | 6.1 |
1.0 | − 0.7 | 0.9 | 1.4 | ND | 0.9 | 5.9 | |
− 1.7 | 0.8 | − 10.0 | 1.0 | ND | ND | 0.4 | |
− 0.2 | − 2.3 | − 13.3 | − 11.3 | ND | ND | ND | |
PAL1 silenced Kaleso plants | − 3.3 | − 7.6 | − 3.3 | − 7.0 | ND | 5.9 | 0.4 |
− 8.0 | − 1.7 | − 0.3 | 2.0 | ND | ND | ND | |
1.1 | 2.5 | 2.7 | 3.4 | ND | ND | ND | |