Skip to main content

Table 2 Amount of infectious virus particles or virus genome copies spiked into the pooled mosquito sample and the total viral load used in the PCR reaction

From: Development of genus-specific universal primers for the detection of flaviviruses

Infectious virus particles/virus genome copies

     

Viral titer in the mosquito homogenate

105.5

104.5

103.5

102.5

101.5

(TCID50/ml)a

     

Viral genome in the mosquito homogenate

107.89

106.72

105.95

104.92

104.20 c

(copies/ml)b

     

Infectious virus load used in the PCR reaction

102.57

101.57

100.57

10–0.43

10–1.43

(TCID50/PCR reaction)d

     

Viral genome used in the PCR reaction

104.95

103.78

103.02

101.99

101.27

(copies/PCR reaction)d

     
  1. aThe JEV vaccine virus was spiked into the 10 mosquito homogenate to yield final concentrations ranging from 105.5–101.5 (tenfold dilutions)
  2. The titer of the spiked virus was determined based on TCID50 (see “Materials and Methods”)
  3. bThe number of copies of the viral genome spiked into the mosquito homogenate was based on the viral genome copy number of cDNAs synthesized from RNA extracted from the JEV vaccine strain (serially diluted from 105.5–100.5 TCID50/ml). The values represent the mean of quadruplicate measurements. The copy number of the viral genome in each cDNA sample was calculated by reading off the cDNA Ct values from a standard curve constructed by plotting the copy number of a plasmid (serially diluted) encoding the JEV genome versus Ct value (see Additional file 1: Fig. S1)
  4. cThis value represents the mean of triplicate measurements (due to the detection limit)
  5. dThe viral load used in the PCR reaction was calculated according to the amount of RNA extracted from the mosquito homogenate and used for cDNA synthesis
Back to article page

Virology Journal

ISSN: 1743-422X