Fig. 5
From: Development of genus-specific universal primers for the detection of flaviviruses
Amplification of the NS5 coding region of JEV from crude mosquito homogenates. NS5 coding sequences were amplified by PCR using cDNA templates derived from crude mosquito homogenates. The mosquito homogenate was prepared from pooled mosquito samples (10 mosquitoes per sample) and then spiked with the serially diluted JEV vaccine strain. The virus titer in the mosquito homogenate was 105.5–101.5 TCID50/ml or zero (no virus). NC: negative control (distilled water). cDNA was synthesized from RNA extracted from an aliquot of whole mosquito fluid. The bands represent amplicons of the NS5 region. The image shown is cropped from the whole gel image